Chemical profiles and metabolite study of raw and processed Cistanche deserticola in rats by UPLC-Q-TOF-MSE

Abstract Background Chinese materia medica processing is a distinguished and unique pharmaceutical technique in Traditional Medicine (TCM) used for reducing side effects, increasing or even changing therapeutic efficacy of the raw herbs.Changes essential components induced by an optimized procedure are primarily responsible increased medicinal plants.The kidney-yang invigorating effect rice wine-steamed Cistancha deserticola ( C. ) was stronger than (CD). Methods A comparison analysis carried out using UPLC-Q-TOF-MS E with UNIFI informatics platform to determine influence processing. In vitro studies were performed characterization constituents as well metabolites vivo. The chemical determined CD its processed products. multivariate statistical analyses conducted evaluate variations between them while OPLS-DA pairwise comparison. Results results this study revealed considerable phenylethanoid glycosides (PhGs) iridoids after total 97 compounds detected extracts product. PhGs having 4'- O -caffeoyl group 8- -?- d -glucopyranosyl part, like acteoside, cistanoside C, campneoside II, osmanthuside decreased being processed, 6'- such isoacetoside, isocistanoside isocampneoside I, isomartynoside increased, especially CD-NP group. intensity echinacoside B whose structure possess moiety also increased. vivo study, 10 prototype 44 rat plasma, feces, urine. obtained that leads variation affected disposition vivo, phase II metabolic processes key cascades each compound most associated acteoside. Conclusions This first global research CD. These findings add our understanding impact give important data future investigations.